A large-sample crisis or not?

A large-sample crisis or not? Exaggerated false positives by popular differential expression methods on human population RNA-seq samples

Sanity check by permutation should be done for large samples

Written by Dr. Yumei Li (UC Irvine) and Jingyi Jessica Li (UCLA)

In biological studies, identifying biological features that are significantly different under different experimental conditions or disease states is important to understand the biological mechanisms behind phenotypes. Among these features, gene expression is the most commonly studied one. …

crisis differential-expression fdr non-parametric permutations rna-seq