Ab-initio Contrast Estimation and Denoising of Cryo-EM Images. (arXiv:2202.07737v1 [cs.CV])

Background and Objective: The contrast of cryo-EM images vary from one to
another, primarily due to the uneven thickness of ice layers. The variation of
contrast can affect the quality of 2-D class averaging, 3-D ab-initio modeling,
and 3-D heterogeneity analysis. Contrast estimation is currently performed
during 3-D iterative refinement. As a result, the estimates are not available
for class averaging and ab-initio modeling. However, these methods require good
initial estimates of 3-D volumes and 3-D rotations of molecules. This …

ab arxiv cryo-em cv images