Ab-initio Contrast Estimation and Denoising of Cryo-EM Images. (arXiv:2202.07737v2 [cs.CV] UPDATED)

Background and Objective: The contrast of cryo-EM images varies from one to
another, primarily due to the uneven thickness of the ice layer. This contrast
variation can affect the quality of 2-D class averaging, 3-D ab-initio
modeling, and 3-D heterogeneity analysis. Contrast estimation is currently
performed during 3-D iterative refinement. As a result, the estimates are not
available at the earlier computational stages of class averaging and ab-initio
modeling. This paper aims to solve the contrast estimation problem directly
from …

ab arxiv cryo-em cv denoising images